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For additional validation, we selected histone H3 lysine 27 acetylation as a result of this modification has not been previously proven to be altered in CRC 19Protein immunostaining on western blots confirmed the elevated K27 acetylation at H3 (Figure 3 ) and immunohistochemical staining of paired CRC and corresponding regular tissue sections revealed nuclear localisation of acetylated H3 protein (Determine four ). |
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